Identification of Food-Derived Collagen Peptides in Human Blood after Oral Ingestion of Gelatin Hydrolysates
Department of Food Sciences and Nutritional Health, Kyoto Prefectural University, 1-5 Shimogamo Kyoto 606-8522, Japan, and Research and Development Center, Nippon Meat Packers, 3-3 Midorigahara, Tsukuba, Ibaraki 300-2646, Japan
- Agric. Food Chem.,2005,53 (16), pp 6531–6536, DOI: 10.1021/jf050206p, Publication Date (Web): July 6, 2005, Copyright © 2005 American Chemical Society
In the present study, we identified several food-derived collagen peptides in human blood after oral ingestion of some gelatin hydrolysates. Healthy human volunteers ingested the gelatin hydrolysates (9.4−23 g) from porcine skin, chicken feet, and cartilage after 12 h of fasting. Negligible amounts of the peptide form of hydroxyproline (Hyp) were observed in human blood before the ingestion. After the oral ingestion, the peptide form of Hyp significantly increased and reached a maximum level (20−60 nmol/mL of plasma) after 1−2 h and then decreased to half of the maximum level at 4 h after the ingestion. Major constituents of food-derived collagen peptides in human serum and plasma were identified as Pro-Hyp. In addition, small but significant amounts of Ala-Hyp, Ala-Hyp-Gly, Pro-Hyp-Gly, Leu-Hyp, Ile-Hyp, and Phe-Hyp were contained.